|
KMID : 0545120190290091488
|
|
Journal of Microbiology and Biotechnology
2019 Volume.29 No. 9 p.1488 ~ p.1493
|
|
|
One-Step Engineering of a Stable, Selectable Marker-Free Autoluminescent Acinetobacter baumannii for Rapid Continuous Assessment of Drug Activity
|
|
Jiang Huofeng
Gao Yamin
Zeng Sheng
Wang Shuai
Cao Zhizhong
Tan Yaoju
Yin Huancai
Liu Jianxiong
Zhang Tianyu
|
|
|
Abstract
|
|
|
|
The rising cases of multidrug-resistant Acinetobacter baumannii (Ab) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/dif system, we constructed a stable, selectable marker-free autoluminescent Ab capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti-Ab drug candidates in vitro.
|
|
|
KEYWORD
|
|
|
Autoluminescent, marker-free, Acinetobacter baumannii, Tn7, Xer/dif recombination system
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|